Res. Agr. Eng., 2017, 63(4):180-186 | DOI: 10.17221/47/2016-RAE

Extremely low frequency electromagnetic field generator suitable for plant in vitro studiesOriginal Paper

Riry Prihatini*,1, Mohamad Puad Abdullah2, Tuan Abdul Rashid bin Tuan Abdullah3, Ismail Said3, Halil Hussin3, Norihan Mohamad Saleh2,4
1 Indonesian Tropical Fruit Research Institute, Solok, Indonesia
2 Department of Cell and Molecular Biology, Faculty Biotechnology and Biomolecular Sciences, University Putra Malaysia, Selangor DE, Malaysia
3 Department of Electrical Engineering, Faculty Engineering, University Tenaga Nasional, Selangor DE, Malaysia
4 Agro-Biotechnology Institute Malaysia, National Institute of Biotechnology Malaysia, Selangor DE, Malaysia

The extremely low frequency electromagnetic field (ELF-EMF) occurs naturally from the earth and artificially as a human invention. The objectives of this study were to develop a suitable ELF-EMF generator for in vitro plants culture studies and to determine the effect of ELF-EMF exposure on in vitro tobacco (Nicotiana tabacum) growth and chlorophyll content. An ELF-EMF generator, the coGEM 1,000 was constructed using four coils of copper wires that were connected to a transformer, multimeter and rheostat. The coGEM 1,000 suitable for tissue culture plants is able to produce stable and uniform 6 and 12 mT 50Hz ELF-EMF in the four coils of the ELF-EMF generator. The tobacco in vitro plantlets were exposed to 6 and 12 mT of 50 Hz ELF-EMF for a period of 0.5, 1, 2 and 4 hours. The exposure to 12 mT ELF-EMF for an hour increased plant growth (shoot height); whereas the exposure to 6 mT Elf-EMF for an hour increased chlorophyll a, chlorophyll b and the total chlorophyll content.

Keywords: chlorophyll content; coGem 1,000; ELF-EMF; growth; tobacco plant

Published: December 31, 2017  Show citation

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Prihatini R, Abdullah MP, Tuan Abdullah TARB, Said I, Hussin H, Mohamad Saleh N. Extremely low frequency electromagnetic field generator suitable for plant in vitro studies. Res. Agr. Eng. 2017;63(4):180-186. doi: 10.17221/47/2016-RAE.
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